TimothyJLeyMD

PubMed

• Molecular responses in decitabine- and decitabine/ venetoclax-treated patients with acute myeloid leukemia and myelodysplastic syndromes.
  Agata Gruszczynska, Abhishek Maiti, Christopher A Miller, Sai Mukund Ramakrishnan, Daniel C Link
  Haematologica. 2024-08-01
• PML::RARA and GATA2 proteins interact via DNA templates to induce aberrant self-renewal in mouse and human hematopoietic cells.
  Casey D S Katerndahl, Olivia R S Rogers, Ryan B Day, Ziheng Xu, Nichole M Helton
  Proceedings of the National Academy of Sciences of the United States of America. 2024-04-30
• 3 citations
  Rapid and accurate remethylation of DNA indeficient hematopoietic cells with restoration of DNMT3A activity.
  Yang Li, Haley J Abel, Michelle Cai, Taylor A LaValle, Tiankai Yin
  Science Advances. 2024-02-02

Journal Articles

• Differences in human ?, ?, and ?-globin gene expression in monkey kidney cells.  
  Humphries, R.K., Ley, T.J., Turner, P., Moulton, A.D., and Nienhuis, A.W, Cell 30:173-183
• RNA processing errors in patients with ? thalassemia.  
  Ley, T.J., Anagnou, N., Pepe, G., and Nienhuis, and A.W, Proc. Natl. Acad. Sci. USA 79:4775-4779
• Spontaneous subarachnoid hemorrhage simulating acute transmural myocardial infarction.  
  Gascon, P., Ley, T., Toltzis, R., and Bonow, R, Am. Heart J. 105:511-513
• 5-azacytidine selectively increases ?-globin synthesis in a patient with ? thalassemia. New Engl.  
  Ley, T.J., DeSimone, J., Anagnou, N.P., Keller, G.H., Humphries, R.K., Turner, P.H., Young, N.S., Heller, P., and Nienhuis, A.W, J. Med. 307:1469-1475
• A weak upstream promoter gives rise to long human -globin RNA molecules.  
  Ley, T.J., and Nienhuis, A.W, Biochem. Biophys. Res. Commun. 112:1041-1048
• 5-azacytidine increases -globin synthesis and reduces the proportion of dense cells in patients with sickle cell anemia.  
  Ley, T.J., DeSimone, J., Noguchi, C., Turner, P., Schechter, A., Heller, P., and Nienhuis, A.W, Blood 62:370-380
• "Silent" nucleotide substitution in a ??-thalassemia gene activates splice site in coding sequence RNA.  
  Goldsmith, M., Humphries, R.K., Ley, T.J., Cline, A., Kantor, J., and Nienhuis, and A.W, Proc. Natl. Acad. Sci. USA 80:2318-2322
• Acquired ?-thalassemia in preleukemia is due to decreased expression of all four ?-globin genes.  
  Anagnou, N.P., Ley, T.J., Chesbro, B., Wright, G., Kitchens, C., Liebhaber, S., Nienhuis, A.W., and Deisseroth, and A.B, Proc. Natl. Acad. Sci. USA 80:6051-6055
• Inducible transcription of five globin genes in K562 human leukemia cells.  
  Dean, A., Ley, T.J., Humphries, R.K., Fordis, M., and Schechter, and A.N, Proc. Natl. Acad. Sci. USA 80:5515-5519
• Human globin gene expression in hybrid 2S MEL x human fibroblast cells.  
  Chiang, Y.L., Ley, T.J., Sanders-Haigh, L., and Anderson, W.F, Somatic Cell and Molecular Genetics 10:399-407
• ?° -39 thalassemia gene: A premature termination codon causes?? mRNA deficiency without affecting cytoplasmic mRNA stability.  
  Humphries, R.K., Ley, T.J., Anagnou, N.P., Baur, A.W., and Nienhuis, A.W, Blood 64:23-32
• DNA methylation and regulation of the human ?-like globin genes in mouse erythroleukemia cells containing human chromosome 11.  
  Ley, T.J., Chiang, Y., Haidaris, D., Anagnou, N.P., Wilson, V., and Anderson, W.F, Proc. Natl. Acad. Sci. USA 81:6618-6622
• Disseminated infection caused by Cunninghamella Bertholletiae in a thalassemia patient receiving 5-azacytidine.  
  Sands, J.M., Macher, A.M., Ley, T.J., and Nienhuis, A.W, Ann. Int. Med. 102:59-63
• 5-azacytidine acts directly on both erythroid precursors and progenitors to increase HbF production.  
  Humphries, R.K., Dover, G.D., Young, N.S., Moore, J., Charache, S., Ley, T.J., and Nienhuis, A.W, J. Clin. Invest 75:547-557
• Fgr -proto-oncogene mRNA induced in B lymphocytes by Epstein-Barr virus infection.  
  Cheah, Marc S.C., Ley, T.J., Tronick, S.R., and Robbins, and K.C, Nature 319:238-240
• Hydroxyurea induction of hemoglobin F production in sickle cell disease: Relationship between cytotoxicity and F cell production.  
  Dover, G.J., Humphries, R.K., Moore, J.G., Ley, T.J., Young, N.S., Charache, S., and Nienhuis, A.W, Blood 67:735-738
• An enhancer element lies 3' to the human A?-globin gene.  
  Bodine, D.M., and Ley, T.J, EMBO J. 6:2997-3004
• High resolution analysis of the human ?-globin gene promoter in K562 erythroleukemia cell chromatin.  
  Gimble, J.M., Max, E.E., and Ley, T.J, Blood 72:606-612
• Tissue-specific expression and developmental regulation of the human fgr-proto-oncogene.  
  Ley, T.J., Connolly, N.L., Katamine, S., Cheah, S.C., Senior, R.M., and Robbins, K.C, Mol. Cell. Biol. 9:92-99
• Globin gene expression in erythroid human fetal liver cells.  
  Ley, T.J., Maloney, K.A., Gordon, J.I., and Schwarz, A.L, J. Clin. Invest. 83:1032-1038
• Genomic organization and chromosomal localization of the human cathepsin G gene.  
  Hohn, P.A., Popescu, N.C., Hanson, R.D., Salvesen, G., and Ley, T.J, J. Biol. Chem. 264:13412-13419
• Analysis of epitope expression and the functional repertoire of recombinant complement receptor 2 (CR2/CD21) in mouse and human cells.  
  Carel, J.-C., Frazier, B., Ley, T.J., and Holers, V.M, J. Immunol. 143:923-930
• Nucleotide sequence and regulation of a human 90-kDa heat shock protein gene.  
  Rebbe, N.F., Hickman, W.S., Ley, T.J., Stafford, D.W., and Hickman, S, J. Biol. Chem. 264:15006-15011
• Developmental regulation of the human cathepsin G gene in myelomonocytic cells.  
  Hanson, R.D., Connolly, N.L., Burnett, D., Campbell, E.J., Senior, R.M., and Ley, T.J, J. Biol. Chem. 265:1524-1530
• A cluster of hematopoietic serine protease genes is found on the same chromosomal band as the human ?????T-cell receptor locus.  
  Hanson, R.D., Hohn, P.A., Popescu, N.C., and Ley, T.J, Proc. Natl. Acad. Sci. USA 87:960-963
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Books/Book Chapters

• Experimental Approaches for the Study of Hemoglobin Switching. 
  Chapter: Augmentation of hemoglobin synthesis by S-phase specific drugs in the K562 cell line 
  Page 205 - 216Dean, A., Wu, Y., Ley, T.J., Fordis, C.M., and Schechter, A.NEditors: G. Stamatoyannopoulos and A.W. Nienhuis.Alan R. Liss, New York
• Annals New York Academy of Sciences. 
  Chapter: Reduced ?-globin gene expression in adult mice containing deletions of locus control region 5' HS-2 or 5' HS-3. 
  Page 445 - 453Ley, T.J., Hug, B., Fiering, S., Epner, E., Bender, M.A., and Groudine, and M
• Matrix Metalloproteinases and Inhibitors. 
  Chapter: Neutral proteinase expression by human mononuclear phagocytes: A prominent role of cellular differentiation 
  Page 363 - 367Welgus, H.G., Senior, R.M., Parks, W.C., Kahn, A.J., Ley, T.J., Shapiro, S.S., and Campbell, E.JGustav Fischer Verlag, Stuttgart, GermanyEdition Supp,
• 7th Hemoglobin Switching Conference Proceedings. 
  Chapter: Conservation of the primary structure, organization, and function of the human and mouse ?-globin locus control regions 
  Page 138 - 152Moon, A.M., and Ley, T.JJohns Hopkins University Press, Baltimore, MD
• Annals New York Academy of Sciences. 
  Chapter: Function of transfected globin promoters and the globin locus activator in K562 erythroleukemia cells 
  Page 207 - 214Ulrich, M.J., Moon, A.M., and Ley, T.J
• Annals New York Academy of Sciences. 
  Chapter: Regulatory regions flanking the human fetal ?-globin genes 565 
  Page 13 - 22Lin, H.J., Bodine, D.M., Rutherford, T.R., Anagnou, N.P., McDonagh, K.T., Ley, T.J., and Nienhuis, and A.W
• Conn's Current Therapy. 
  Chapter: Thalassemia 
  Page 311 - 315Ley, T.JEditors: R.E. Rakel.W.B. Saunders Co., Philadelphia, PA
• Conn's Current Therapy. 
  Chapter: Thalassemia 
  Page 307 - 312Ley, T.JEditors: R.E. Rakel.W. B. Saunders Co., Philadelphia, PA
• Developmental Control of Globin Gene Expression. 
  Chapter: A transient assay system to study ?-globin gene expression in K562 cells 
  Page 189 - 200Bodine, D.M., and Ley, T.JAlan R. Liss, New York
• Clinics in Hematology. 
  Chapter: Transfusion hemosiderosis and chelation therapy 
  Page 437 - 464Ley, T.J., Griffith, P., and Nienhuis, A.WW.B. Saunders Co, London
• Experimental Approaches for the Study of Hemoglobin Switching. 
  Chapter: Pharmacologic manipulation of fetal hemoglobin synthesis 
  Page 447 - 454Dover, G.J., Humphries, R.K., Young, N.S., Ley, T.J., Boyer, S., Charache, S., and Nienhuis, and A.WEditors: G. Stamatoyannopoulos and A.W. Nienhuis.Alan R. Liss, New York
• Principles and Techniques of Human Research and Therapeutics. 
  Chapter: Prospects for therapeutic manipulation of human gene function 
  Page 59 - 85Ley, T.J., and Nienhuis, A.WEditors: F.G. McMahon and T.J. Ley.Futura, Mt. Kisco, New YorkEdition Vol.,
• Globin Gene Expression and Hematopoietic Differentiation. 
  Chapter: A silent nucleotide substitution activates a cryptic splice site in coding sequence RNA 
  Page 123 - 126Humphries, R.K., Ley, T.J., Goldsmith, M., Cline, A., Kantor, J.A., and Nienhuis, A.WEditors: G. Stamatoyannopoulos and A.W. Nienhuis.Alan R. Liss, New York
• Globin Gene Expression and Hematopoietic Differentiation. 
  Chapter: Globin gene expression in somatic cell hybrids 
  Page 39 - 52Anderson, W.F., Chiang, Y.L., Sanders-Haigh, L., and Ley, T.JEditors: G. Stamatoyannopoulos and A.W. Nienhuis.Alan R. Liss, New York
• Globin Gene Expression and Hematopoietic Differentiation. 
  Chapter: A. Abundance and structure of globin mRNAs in K562 human leukemia cells 
  Page 323 - 335Dean, A., Ley, T.J., Humphries, R.K., Fordis, C.M., and SchechterEditors: G. Stamatoyannopoulos and A.W. Nienhuis.Alan R. Liss, New York
• Globin Gene Expression and Hematopoietic Differentiation. 
  Chapter: Globin gene methylation and expression in patients treated with 5-azacytidine 
  Page 457 - 474Ley, T.J., Anagnou, N.P., Turner, P., DeSimone, J., Heller, P., and Nienhuis, A.WEditors: G. Stamatoyannopoulos and A.W. Nienhuis.Alan R. Liss, New York
• Proceedings of the Regulation of Hemoglobin Biosynthesis. 
  Chapter: Expression of human normal and thalassemic globin genes 
  Page 385 - 389Nienhuis, A.W., Ley, T.J., and Humphries, R.KEditors: E. Goldwasser.Elsevier, North Holland
• March of Dimes - Birth Defects Foundation. 
  Chapter: Thalassemia: Recent advances in detection and treatment 
  Page 69 - 79Nienhuis, A.W., Ley, T.J., Pepe, G., Tam, J., and Kantor, and JEditors: Cao, Antonio, Carcassi, Ugo, and P.T. Rowley.Alan R. Liss, Inc. , New YorkEdition Vol.,
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Abstracts/Posters

• The Molecular Basis of Long First Remissions in Normal Karyotype AML Patients
  Timothy J Ley, 61st Annual American Society of Hematology Meeting, Orlando, FL, 12/9/2019
• Whole Genome Bisulfite Sequencing of 63 Primary AML Samples Identifies a Unique DNA Hypermethylation Signature for Mutant IDH1/2 Cases That Is Different from That of T...
  Timothy J Ley, 61st Annual American Society of Hematology Meeting, Orlando, FL, 12/9/2019
• Gata2 Expression and Function in PML-RARA-driven Preleukemia and APL
  Timothy J Ley, 61st Annual American Society of Hematology Meeting, Orlando, FL, 12/9/2019

Lectures

• Evidence for Complete Mutation Clearance in Normal Karyotype AML Patients with Very Long (> 5 years) Remissions after Chemotherapy Alone 
  2018 ASH Annual Meeting - San Diego, CA - 12/1/2018
• Direct Detection of Expressed Mutations in AML Cells Using Single Cell RNA-Sequencing, and Its Impact on Defining Sources of Expression Heterogeneity 
  2018 ASH Annual Meeting - San Diego, CA - 12/1/2018
• Basic Science Symposium on AML Genomics. 
  American Society of Hematology
• Cancer Consortium Symposium. 
  Fred Hutchinson Cancer Research Center
• Gaffney Memorial Lecture. 
  University of Pittsburgh Children’s Hospital
• Kierkow Memorial Lecture. 
  Northwestern University
• Distinguished Scientist Seminar Series. 
  University of Cincinnati
• Institute for Genomic Medicine Inaugural Symposium. 
  UCSD
• Cancer Center Seminar Series. 
  Terry Fox Laboratory, University of British Columbia, 1999, 2006, 2010
• Leukemia Retreat Keynote Speaker. 
  UCSF
• Biology Seminar Series. 
  Drake University
• Lewis-Sigler Institute Seminar Series. 
  Princeton University
• Benning Society Seminar Series. 
  University of Utah
• Dept of Biochemistry & Molecular Genetics Red Banner Seminar Series. 
  University of Alabama, Birmingham, AL
• Distinguished Lecturer Series. 
  University of Oklahoma Department of Biochemistry
• The Broad Institute. 
  Genomics of Common Disease Symposia
• Seminars in Biomedical Sciences. 
  UCSF
• Immunology Seminar Series. 
  University of Miami, 2005, 2006
• Immunology Scientific Symposium. 
  American Society of Hematology, 1994
• Cytotoxic T cells Symposium. 
  International Congress of Immunology
• Janet Rowley Symposium. 
  University of Chicago
• American Society of Investigative Dermatology. 
  Herman Beerman Lecture and Award
• Basic Science Seminar Series. 
  Merck Pharmaceuticals, 2000; Blood Center, Harvard University, 2002; Siteman Cancer Center, 2004
• Cytotoxic Lymphocyte Biology Symposium and Plenary Talk. 
  International Congress of Immunology
• Tinsley Randolf Harrison Society Lectureship. 
  Vanderbilt University
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