LawrenceGreenfieldMDPh.D., F.A.C.B.

PubMed

• 41 citations
  DNA sequence of the araBAD promoter in Escherichia coli B/r.
  Lawrence Greenfield, Thomas C. Boone, Gary Wilcox
  Proceedings of the National Academy of Sciences of the United States of America. 1978-10-01
• 15 citations
  Hybrid plasmids containing the araBAD genes of Escherichia coli B/r.
  Donald A. Kaplan, Lawrence Greenfield, Thomas C. Boone, Gary Wilcox
  Gene. 1978-05-01

Journal Articles

• Antibody-DT Conjugates in the Study of Surface Antigen Properties and DT Cytotoxic Mechanism.  
  Colombatti, M., Dell’Arciprete, L., Greenfield, L., Youle, R.J., Rappuoli, R., and Tridente, G, Bakteriol. Mikrobiol. Hyg. ABT
• Conversion of closed circular DNA molecules to single nicked molecules by digestion with DNase I in the presence of ethidium bromide.  
  Greenfield, L., Simpson, L. and Kaplan, D, Biochim. Biophys. Acta
• The araC regulatory gene mRNA contains a leader sequence.  
  Cass, L., Horwitz, A., Miyado, G., Greenfield, L. and Wilcox, G, Molec. Gen. Genetics
• Chromogenic method to screen very large populations of bacteriophage plaques for the presence of specific antigen.  
  Kaplan, D.A., Greenfield, L. and Collier, R.J, Methods in Enzymology
• Nucleotide sequence of the structural gene for diphtheria toxin carried by corynebacteriophage beta.  
  Greenfield, L., Bjorn, M.J., Horn, G., Fong, D., Buck, G.A., Collier, R.J. and Kaplan, D.A, Proc. Natl. Acad. Sci
• Cloned fragment of diphtheria toxin linked to T cell specific antibody identifies regions of B chain active in cell entry.  
  Colombatti, M., Greenfield, L. and Youle, R.J, J. Biol. Chem
• High level expression of diphtheria toxin peptides in Escherichia coli.  
  Greenfield, L., Dovey, H.F., Lawyer, F.C. and Gelfand, D.H, Bio/technology
• Functional expression of the human transferrin receptor cDNA in chinese hamster ovary cells deficient in endogenous transferrin receptor.  
  McGraw, T.E., Greenfield, L. and Maxfield, F.R, J. Cell Biology
• Point Mutations in diphtheria toxin can separate binding from entry and amplify immunotoxin selectivity.  
  Greenfield, L., Johnson, V.G., and Youle, R.J, Science
• Immunotoxin Therapy for Leptomeningeal Neoplasma.  
  Laske, D.W., Muraszko, K. M., Oldfield, E. H., DeVroom, H. L., Sung, C., Dedrick, R. L., Simon, T. R., Colandrea, J., Copeland, C., Katz, D., Greenfield, L., Groves, E..., Neurosurgery
• The role of the diphtheria toxin receptor in cytosol translocation.  
  Johnson, V.G., Wilson, D., Greenfield, L., and Youle, R.J, Journal of Biological Chemistry
• Improved immunotoxins engineered for increased tumor specificity: potential for treatment of central nervous system tumors and leptomeningeal neoplasia.  
  Johnson, V.G., Wrobel, C., Wilson, D., Zovickian, J., Greenfield, L., Oldfield, E.H., and Youle, R, J. of Neurosurgery
• A preliminary pharmacokinetic study of 111In-labeled 260F9 anti-(breast cancer) antibody in patients.  
  Griffin, T.W., Bokhari, F., Collins, J., Stochi, M., Bernier, M., Gionet, M., Siebecker, D., Wertheimer, M., Groves, E.S., Greenfield, L., Houston, L.L., Doherty, P.W...., Cancer Immunol. Immunother
• Thiol-Containing Cross-Linking Agent with Enhanced Steric Hindrance.  
  Greenfield, L., Bloch, W., and Moreland, M, Bioconjugate Chemistry
• Effect of Coupling Chemistry on the Pharmacology of Recombinant Ricin A Chain Immunotoxins.  
  Greenfield, L., and Dovey, H, Antibody, Immunoconjugates and Radiopharmaceuticals
• Pharmacokinetics and Toxicology of Immunotoxins Administered Into the Subarachnoid space in Nonhuman Primates and Rodents.  
  Muraszko,K., Sung,C., Walbridge, S., Greenfield, L., Dedrick, R.L., Oldfield, E.H., and Youle, R.J, Cancer Research
• Rapid and Simple PCR Assay for Quantitation of Human Immunodeficiency Virus Type 1 RNA in Plasma: Application to Acute Retroviral Infection.  
  Mulder, J., McKinney, N., Christopherson, C., Sninsky, J., Greenfield, L., and Kwok, S, J. Clin. Micro
• Reactivity of Murine Cytokine Fusion Toxin, Diphtheria Toxin390-Murine Interleukin-3 (DT390-mIL-3), With Bone Marrow Progenitor Cells.  
  Chan, C-H., Blazar, B.R., Greenfield, L., Kreitman R.J., and Vallera, D. A, Blood
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Books/Book Chapters

• Sample Preparation Methods. in Diagnostic Molecular Microbiology: Principles and Applications. 
  Greenfield, L., and White, TEditors: Persing, D. H., Smith, T. F., Tenover, F. C., and White, T. J.American Society for Microbiology, Washington, D.C
• The Role of the CrossLinking Reagent in the Behavior Immunotoxins in Vitro and in Vivo. 
  Chapter: Therapeutic Proteins: Pharmacokinetics and Pharmacodynamics 
  Page 91 - 140Greenfield, LEditors: Kung, A. H. C., Baughman, R. A. and Larrick, J. W..Freeman and Company, New York: W. H
• Chemical and Genetic Characterization of the Enzymatic Activity Associated with Ricin A Chain. 
  Chapter: Genetically Engineered Toxins 
  Page 151 - 182Greenfield, LEditors: Frankel, A.Marcel Dekker, Inc, New York
• Genetics of Diphtheria Toxin Expression in Corynebacterium diphtheriae. 
  Chapter: Genetically Engineered Toxins 
  Page 273 - 305Greenfield, LEditors: Frankel, A.Marcel Dekker, Inc, New York
• Genetics of Diphtheria Toxin Production and Expression in Escherichia coli. 
  Chapter: Genetically Engineered Toxins 
  Page 307 - 338Greenfield, LEditors: Frankel, A.Marcel Dekker, Inc, New York
• Cancer Treatment and Research: Immunotoxins 
  Chapter: Genetic engineering of immunotoxins 
  Page 113 - 112Youle, R.J., Greenfield, L., and Johnson, V.GEditors: A.E. Franke.Martinus Nijhoff Publishing, Boston, Mass
• The appropriate toxicologic testing of recombinant human proteins. 
  Chapter: Banbury Report 29: Therapeutic Peptides and Proteins: Assessing the New Technologies 
  Page 175 - 187Marafino, B.J., Young, J.D., Greenfield, I.L., and Kopplin, J.Rcold spring harbor laboratory press
• Antibody toxin conjugates help clarify surface antigen behavior and toxin entry mechanism. 
  Chapter: Mediated Cytotoxicity and Cellular Biology, New Series 
  Page 115 - 165Colombatti, M., Greenfield, L., and Youle, R.JEditors: B. Bonavida, and R.J. Collier.Alan R. Liss, Inc, New York, N.Y
• Studies on the controlling site region of the araBAD operon of Escherichia coli B/r. 
  Chapter: Ribosomes and Nucleic Acid Metabolism 
  Page 287 - 299Wilcox, G., Greenfield, L. and Boone, TEditors: J. Zelinka and J. Balam.Publishing House of the Slovak Academy of Sciences
• Molecular cloning of segments of the M13 genome. 
  Chapter: The Single Stranded Bacteriophage 
  Page 461 - 466Kaplan, D.A., Greenfield, L. and Wilcox, GEditors: D. Denhardt, D. Dressler, and D. Ray.Cold Spring Harbor Laboratory Press
• Molecular cloning of the h80dara restriction fragments with noncomplementary ends. 
  Chapter: Molecular Approaches to Eucaryotic Genetic Systems 
  Page 85 - 102Kaplan, D.A., Greenfield, L. and Wilcox, GEditors: Wilcox, J. Abelson and C. F. Fox.Academic Press, New York
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Abstracts/Posters

• Quantitation of HIV-1 Plasma Viremia by PCR with Tth DNA Polymerase.
  Christopherson, C., Mulder, J., McKinney, N., Greenfield, L., Schwartz, R., Russell, M., Spahlinger, T., Jackson, J. B., and Kwok, ICAAC
• Quantitative introduction of single nicks into closed kinetoplast DNA minicircles.
  Greenfield, L. and Simpson, L, 14th Annual Meeting American Society of Cell Biology
• Molecular cloning of restriction fragments of lambda h80dara DNA.
  Greenfield, L., Kaplan, D.A. and Wilcox, G, Journal of Supramolecular Structure
• Cloned diphtheria toxin fragments of improved efficacy in immunoconjugates.
  Greenfield, L., Nitecki, D., Scalapino, L., Groetsema, G., and Bjorn, M, Gordon Conference on Drug Carriers in Medicine and Biology, Plymouth, New Hampshire
• Ways to improve the in vitro cytotoxicity of immunotoxins.
  Frankel, A., Ring, D., Greenfield, L. and Bjorn, M, University of London Meeting of Advances in the Applications of Monoclonal Antibodies
• Cloned diphtheria toxin fragments of improved efficacy in immunoconjugates.
  Greenfield, L., Scalapino, L., Groetsema, G. and Bjorn, M, Second Nature Conference: The Molecular Biology of Cancer, Boston, Massachusetts
• Enzymatically inactive diphtheria A fragment: expression E. coli and toxicity characterization.
  Emerick, A., Greenfield, L. and Gates, C, DNA
• Cloned diphtheria toxin fragments of improved efficacy in immunoconjugates.
  Greenfield, L., Nitecki, D., Scalapino, L., Groetsema, G. and Bjorn, M, DNA
• Expression of the human transferrin receptor in a chinese hamster ovary cell line deficient in endogenous transferrin receptor.
  McGraw, T.E., Greenfield, L. and Maxfield, F.R, J. of Cell Biology
• Engineering of diphtheria toxin for use in immunoconjugates.
  Greenfield, L. and Dovey, R, J. of Cellular Biochemistry
• Point mutations in diphtheria toxin separate binding from entry.
  Johnson, V.G., Greenfield, L., Wilson, D.J., and Youle, R.J, 27th Annual meeting of the American Society for Cell Biology, S. Louis, Missouri
• Point mutations in diphtheria toxin separate binding from entry and amplify immunotoxin selectivity.
  Johnson, V.G., Greenfield, L., Wilson, D., Wrobel, C., and Youle, R, presented at the 3rd International Conference on monoclonal antibody immunoconjugates for cancer, San Diego, California
• Pharmacokinetics and efficacy of immunotoxins containing recombinant ricin A chain.
  Greenfield, L., Bell, D., Apell, G., and Young, J, International Symposium on immunotoxins, Duke University, Durham, North Carolina
• Modifications of Diphtheria toxin for use in immunotoxins.
  Greenfield, L., Johnson, V.J., Colombatti, M., and Youle, R, Society for Industrial Microbiology, Chicago, Illinois
• A Simple Method for the Detection and Quantitation of Low Amounts of a Specific DNA Target Utilizing the Polymerase Chain Reaction.
  Greenfield, L., Groetsema, G., Watson, R., Holland, P. M., Will, S., and Bloch, W, 203rd American Chemical Society National Meeting, San Francisco
• A PCR-based Assay That Utilizes the Inherent 5' of rTth DNA Polymerase for the Quantitative Detection of HCV Detection of HCV RNA.
  Tsang, S.Y., Kwok, S., and Greenfield, L, ASM Conference on Molecular Diagnostics and Therapeutics, Moran, Wyo
• OncoScan™ FFPE Express 2.0: A Cancer-Focused Whole-Genome Copy Number Platform Requiring only 75-ng of Input DNA from FFPE Samples (OncoScan™ FFPE Services.
  Wang, Y., Sapolsky, R., Venkatapathy, S., Siddiqui, F., Shen, F., Thompson, P., Campbell, I., Stengel, G., Estabrook, A., Shukla, A., Weaver, P., Wai, W., Huynh, V., a..., AACR 2012 Annual Meeting, Chicago, Illinois
• Methods for Rendering Mycobacteria in Clinical Specimens Non-Viable.
  Young, K., Mohr, K., Dodge, D., Greenfield, L., and Koenig, G, American Society for Microbiology
• Automated Nucleic Acid Sample Preparation For Genomics Research.
  Montesclaros, L., Felton, A., Voice, R., Somack, R., Gentile, A., Bi, W., Borodkin, M., Hosizaki, J., Livak, K., Luo, Q., Mirchandani, V., Reed, M., Sandell, D., Sande..., Lab Automation, Palm Springs, California
• Automated Total RNA Extraction From Whole Blood Using The ABI Prism 6700 Workstation.
  Tanner, M., Felton, A., Voice, R., Greenfield, L., Ruff, D., and Snider, J, 92nd Annual Meeting of the American Association for Cancer Research, New Orleans, Louisiana
• Automated Total RNA Extraction From Whole Blood Using The ABI Prism 6700 Workstation.
  Tanner, M., Felton, A., Voice, R., Greenfield, L., Ruff, D., and Snider, J, Genomic Sample Preparation Conference, Boston, Massachusetts
• Automated Nucleic Acid Sample Preparation For Genomic Research.
  Montesclaros, L., Felton, A., Voice, R., Somack, R., Gentile, A., Bi, W., Borodkin, M., Hosizaki, J., Livak, K., Luo, Q., Mirchandani, V., Sandell, D., Sandell, S., Th..., Genomic Sample Preparation Conference, Boston, Massachusetts
• Fluo-4 NW: A New No Wash Calcium Assay for HTS.
  Dzubay, J., Schulenberg, B., Martin, V., Antoulinakis, E., Berlier, J., Hoffman, R., Greenfield, L., Johnson, I, Drug Discovery Technology and Development, Boston, MA
• Optical Diagnostic Imaging From Bench to Bedside at the National Institute of Health.
  Greenfield, L, Optical Imaging 2006 Fifth Inter-Institute Workshop, Bethesda, Maryland
• Multiplex In vivo Imaging: Imaging Multiple Targets To Gain Spatial And Temporal Context Of the In Vivo Events.
  Greenfield, L, Symposium for In Vivo Small Animal Imaging, Northwestern University. Chicago, IL
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Other

• Composition and Methods of Selective Nucleic Acid Isolation. 
  Bost, D. and Greenfield, L, US 20100021905
• Compositions and Methods of Selective Nucleic Acid Isolation. 
  Bost, D. and Greenfield, L, Patent Number 7,537,898
• Compositions, Methods, and Kits for Isolating Nucleic Acids Using Surfactants and Proteases. 
  Greenfield, L. and Monteslaros, L, Patent Number 7,303,876
• Fluorescent Polarization Assay. 
  Sekar, M. M. A. and Greenfield, I. L, Patent Number 7,217,518
• Compositions And Methods Of Selective Nucleic Acid Isolation. 
  Bost, D.A. and Greenfield, L, Patent Number 7,208,271
• Compositions, Methods, and Kits For Isolating Nucleic Acids Using Surfactants and Proteases. 
  Greenfield, I.L, Patent Number 7,001,724
• Compositions, Methods, And Kits For Isolating Nucleic Acids Using Surfactants and Proteases. 
  Greenfield, L. and Montesclaros, L, Patent Number 6,762,027
• ) Purified Thermostable Nucleic Acid Polymerase Enzyme From Thermosipho africanus. 
  Gelfand, D. H., Greenfield, I. L., and Reichert, F. L, Patent Number 5,968,799
• DNA Encoding Thermostable Nucleic Acid Polymerase Enzyme From Thermus Species ZO5. 
  Abramson, R. D., Gelfand, D. H., Greenfield, I. L
• Mutant Diphtheria Toxin Conjugates. 
  Johnson, V. G., Greenfield, L., Laird, W., and Youle, R, Patent Number 5,792,458
• Stable Compositions Comprising Chromogenic Compounds and Methods of Use. 
  Greenfield, L., Starkenburg, S., Shallice, M., Nyhus, J., and Leong, L, US 20120077211
• Optically-Detectable Enzyme Substrates and Their Method of Use. 
  Corry, S.B., Downey, W.L., Filanoski, B., Gee, K.R., Greenfield, I.L., Hirsch, J.D., Johnson, I.; and Rukavishnikov, A, US20120040380
• Methods for Selecting Binding Moieties. 
  Greenfield, L, US 20100240547
• Method for Generating A Predetermined Amount of Nucleic Acid. 
  Greenfield, I.L. and Bost, D.A, Patent Application Number WO2005056836
• Control of Chemical Modification. 
  Mauro, J.M., Steinberg, T.H., Greenfield, L.I. and Leong, L, US 20100009342
• Optically-Detectable Enzyme Substrates and Their Methods of Use. 
  Corry, S.B., Downey, W.L., Filanoski, B., Gee, K.R., Greenfield, I.L., Hirsch, J.D., Johnson, I., and Rukavishnikov, A, Patent ApplicationUS 20090047692
• Control of Chemical Modification. 
  Mauro, J.M., Steinberg, T.H., Greenfield, L.I., and Leong, L, US 20080176213
• Composition Method and Kit for Reducing Background Staining. 
  Filanoski, B., Greenfield, I.L. and Hirsch, J.D, Patent Application US 20080038773
• Mutated Thermostable Nucleic Acid Polymerase Enzyme from Thermus Species Z05. 
  Abramson, R., Gelfand, D.H., and Greenfield, L, Patent Number 5,455,170
• DNA Encoding A Mutated Thermostable Nucleic Acid Polymerase Enzyme from Thermus Species SPS17. 
  Abramson, R., Gelfand, D.H., and Greenfield, L, Patent Number 5,405,774
• Method of Preparing Diphtheria Immunotoxins. 
  Johnson, V.G., Greenfield, L., Youle, R.J., and Laird, W, Patent Number 5,208,021
• Coupling Agents and Sterically Hindered Disulfide Linked Conjugates Prepared Therefrom. 
  Greenfield, I. L., Moreland, M., and Nitecki, D, Patent Number 5,241,078
• Use of a Modified Soluble Pseudomonas Exotoxin A in Immunoconjugates. 
  Greenfield, I. L, Patent Number 4,933,288
• Recombinant Diphtheria Toxin Fragments. 
  Gelfand, D.H., Greenfield, L.I. and Lawyer, F.C, Patent Number 4,830,962
• Toxin Conjugates. 
  Greenfield, I. L., Kaplan, A. D., and Nitecki, D. E, Patent Number 4,894,443
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